diff --git a/src/benchmarks/micro/MicroBenchmarks.csproj b/src/benchmarks/micro/MicroBenchmarks.csproj
index 56be2af97cc..86dc94605ba 100644
--- a/src/benchmarks/micro/MicroBenchmarks.csproj
+++ b/src/benchmarks/micro/MicroBenchmarks.csproj
@@ -111,7 +111,9 @@
 
   <ItemGroup Condition=" '$(TargetFrameworkIdentifier)' == '.NETFramework' Or ('$(TargetFrameworkIdentifier)' == '.NETCoreApp' And '$(_TargetFrameworkVersionWithoutV)' &lt; '6.0')">
     <Compile Remove="libraries\System.Collections\PriorityQueue\Perf_PriorityQueue.cs" />
+    <Compile Remove="libraries\Common\AlignedMemory.cs" />
     <Compile Remove="libraries\System.IO.FileSystem\Perf.RandomAccess.cs" />
+    <Compile Remove="libraries\System.IO.FileSystem\Perf.RandomAccess.NoBuffering.cs" />
   </ItemGroup>
 
   <ItemGroup>
diff --git a/src/benchmarks/micro/libraries/Common/AlignedMemory.cs b/src/benchmarks/micro/libraries/Common/AlignedMemory.cs
new file mode 100644
index 00000000000..a7188984785
--- /dev/null
+++ b/src/benchmarks/micro/libraries/Common/AlignedMemory.cs
@@ -0,0 +1,86 @@
+// Licensed to the .NET Foundation under one or more agreements.
+// The .NET Foundation licenses this file to you under the MIT license.
+// See the LICENSE file in the project root for more information.
+
+using System.Buffers;
+using System.Memory;
+using System.Runtime.CompilerServices;
+using System.Runtime.InteropServices;
+using System.Threading;
+
+namespace System
+{
+    internal sealed class AlignedMemory : MemoryManager<byte>
+    {
+        private bool _disposed;
+        private int _refCount;
+        private IntPtr _memory;
+        private int _length;
+
+        private unsafe AlignedMemory(void* memory, int length)
+        {
+            _memory = (IntPtr)memory;
+            _length = length;
+        }
+
+        public static unsafe AlignedMemory Allocate(nuint length, nuint alignment)
+            => new AlignedMemory(NativeMemory.AlignedAlloc(length, alignment), (int)length);
+
+        public bool IsDisposed => _disposed;
+
+        public unsafe override System.Span<byte> GetSpan() => new System.Span<byte>((void*)_memory, _length);
+
+        public override MemoryHandle Pin(int elementIndex = 0)
+        {
+            unsafe
+            {
+                Retain();
+                if ((uint)elementIndex > _length) throw new ArgumentOutOfRangeException(nameof(elementIndex));
+                void* pointer = Unsafe.Add<byte>((void*)_memory, elementIndex);
+                return new MemoryHandle(pointer, default, this);
+            }
+        }
+
+        private bool Release()
+        {
+            int newRefCount = Interlocked.Decrement(ref _refCount);
+
+            if (newRefCount < 0)
+            {
+                throw new InvalidOperationException("Unmatched Release/Retain");
+            }
+
+            return newRefCount != 0;
+        }
+
+        private void Retain()
+        {
+            if (_disposed)
+            {
+                throw new ObjectDisposedException(nameof(AlignedMemory));
+            }
+
+            Interlocked.Increment(ref _refCount);
+        }
+
+        protected override unsafe void Dispose(bool disposing)
+        {
+            if (_disposed)
+            {
+                return;
+            }
+
+            NativeMemory.AlignedFree(_memory.ToPointer());
+            _disposed = true;
+        }
+
+        protected override bool TryGetArray(out ArraySegment<byte> arraySegment)
+        {
+            // cannot expose managed array
+            arraySegment = default;
+            return false;
+        }
+
+        public override void Unpin() => Release();
+    }
+}
\ No newline at end of file
diff --git a/src/benchmarks/micro/libraries/System.IO.FileSystem/Perf.RandomAccess.NoBuffering.cs b/src/benchmarks/micro/libraries/System.IO.FileSystem/Perf.RandomAccess.NoBuffering.cs
new file mode 100644
index 00000000000..f3d9a2c0961
--- /dev/null
+++ b/src/benchmarks/micro/libraries/System.IO.FileSystem/Perf.RandomAccess.NoBuffering.cs
@@ -0,0 +1,117 @@
+// Licensed to the .NET Foundation under one or more agreements.
+// The .NET Foundation licenses this file to you under the MIT license.
+// See the LICENSE file in the project root for more information.
+
+using System.Buffers;
+using System.Collections.Generic;
+using System.Linq;
+using System.Runtime.CompilerServices;
+using System.Runtime.InteropServices;
+using System.Threading;
+using System.Threading.Tasks;
+using BenchmarkDotNet.Attributes;
+using BenchmarkDotNet.Extensions;
+using MicroBenchmarks;
+using Microsoft.Win32.SafeHandles;
+
+namespace System.IO.Tests
+{
+    [BenchmarkCategory(Categories.Libraries, Categories.NoWASM)]
+    [OperatingSystemsFilter(allowed: true, platforms: OS.Windows)] // NO_BUFFERING is supported only on Windows: https://github.com/dotnet/runtime/issues/27408
+    public class Perf_RandomAccess_NoBuffering
+    {
+        private const int OneKibibyte  = 1 << 10; // 1024
+        private const int OneMibibyte = OneKibibyte  << 10;
+        private const int HundredMibibytes = OneMibibyte * 100;
+        private const FileOptions NoBuffering = (FileOptions)0x20000000;
+        private const FileOptions AsyncNoBuffering  = FileOptions.Asynchronous | NoBuffering;
+
+        static int PageSize = Environment.SystemPageSize;
+
+        private Dictionary<long, string> _sourceFilePaths, _destinationFilePaths;
+        private Dictionary<int, AlignedMemory[]> _countToAlignedMemory;
+        private Dictionary<int, System.Memory<byte>[]> _countToMemory;
+        private Dictionary<int, System.ReadOnlyMemory<byte>[]> _countToReadOnlyMemory;
+
+        private void Setup(params long[] fileSizes)
+        {
+            _countToAlignedMemory = new Dictionary<int, AlignedMemory[]>()
+            {
+                { 4, Enumerable.Range(0, 4).Select(_ => AlignedMemory.Allocate((nuint)PageSize, (nuint)PageSize)).ToArray() },
+                { 16, Enumerable.Range(0, 16).Select(_ => AlignedMemory.Allocate((nuint)PageSize, (nuint)PageSize)).ToArray() },
+            };
+            _countToMemory = _countToAlignedMemory.ToDictionary(pair => pair.Key, pair => pair.Value.Select(aligned => aligned.Memory).ToArray());
+            _countToReadOnlyMemory = _countToAlignedMemory.ToDictionary(pair => pair.Key, pair => pair.Value.Select(aligned => (System.ReadOnlyMemory<byte>)aligned.Memory).ToArray());
+
+            _sourceFilePaths = fileSizes.ToDictionary(size => size, size => CreateFileWithRandomContent(size));
+            _destinationFilePaths = fileSizes.ToDictionary(size => size, size => CreateFileWithRandomContent(size));
+
+            static string CreateFileWithRandomContent(long fileSize)
+            {
+                string filePath = FileUtils.GetTestFilePath();
+                File.WriteAllBytes(filePath, ValuesGenerator.Array<byte>((int)fileSize));
+                return filePath;
+            }
+        }
+
+        [GlobalCleanup]
+        public void Cleanup()
+        {
+            foreach (string filePath in _sourceFilePaths.Values.Concat(_destinationFilePaths.Values))
+            {
+                File.Delete(filePath);
+            }
+            foreach (IDisposable alignedMemory in _countToAlignedMemory.Values.SelectMany(buffers => buffers))
+            {
+                alignedMemory.Dispose();
+            }
+        }
+
+        [GlobalSetup(Targets = new[] { nameof(ReadScatterAsync), nameof(WriteGatherAsync) })]
+        public void SetupBigFileBenchmarks() => Setup(OneMibibyte, HundredMibibytes);
+
+        public IEnumerable<object[]> ReadWrite_MultipleBuffers_Arguments()
+        {
+            yield return new object[] { OneMibibyte, 4 }; // medium size file, 4xpage size user buffers
+            yield return new object[] { OneMibibyte, 16 }; // medium size file, 16xpage size user buffers
+            yield return new object[] { HundredMibibytes, 4 }; // big file, 4xpage size user buffers
+            yield return new object[] { HundredMibibytes, 16 }; // big file, 16xpage size user buffers
+        }
+
+        [Benchmark]
+        [ArgumentsSource(nameof(ReadWrite_MultipleBuffers_Arguments))]
+        public async Task<long> ReadScatterAsync(long fileSize, int count)
+        {
+            CancellationToken cancellationToken = CancellationToken.None;
+            System.Memory<byte>[] buffers = _countToMemory[count];
+            long bytesRead = 0;
+            using (SafeFileHandle fileHandle = File.OpenHandle(_sourceFilePaths[fileSize], FileMode.Open, FileAccess.Read, FileShare.Read, AsyncNoBuffering))
+            {
+                while (bytesRead < fileSize)
+                {
+                    bytesRead += await RandomAccess.ReadAsync(fileHandle, buffers, bytesRead, cancellationToken);
+                }
+            }
+
+            return bytesRead;
+        }
+
+        [Benchmark]
+        [ArgumentsSource(nameof(ReadWrite_MultipleBuffers_Arguments))]
+        public async Task WriteGatherAsync(long fileSize, int count)
+        {
+            CancellationToken cancellationToken = CancellationToken.None;
+            System.ReadOnlyMemory<byte>[] buffers = _countToReadOnlyMemory[count];
+            long buffersSize = count * PageSize;
+            using (SafeFileHandle fileHandle = File.OpenHandle(_destinationFilePaths[fileSize], FileMode.Create, FileAccess.Write, FileShare.Read, AsyncNoBuffering))
+            {
+                long bytesWritten = 0;
+                for (int i = 0; i < fileSize / buffersSize; i++)
+                {
+                    await RandomAccess.WriteAsync(fileHandle, buffers, bytesWritten, cancellationToken);
+                    bytesWritten += buffersSize;
+                }
+            }
+        }
+    }
+}
\ No newline at end of file