diff --git a/eng/pipelines/coreclr/exploratory.yml b/eng/pipelines/coreclr/exploratory.yml
index 7b094398a0f3de..e815ea6260d3c3 100644
--- a/eng/pipelines/coreclr/exploratory.yml
+++ b/eng/pipelines/coreclr/exploratory.yml
@@ -40,7 +40,7 @@ extends:
             buildArgs: -s clr+libs -c $(_BuildConfig) -lc Release
             timeoutInMinutes: 360
             postBuildSteps:
-              - script: $(Build.SourcesDirectory)/src/tests/build$(scriptExt) $(_BuildConfig) $(archType) $(crossArg) generatelayoutonly /p:UsePublishedCrossgen2=false
+              - script: $(Build.SourcesDirectory)/src/tests/build$(scriptExt) $(_BuildConfig) $(archType) $(crossArg) generatelayoutonly
                 displayName: Create Core_Root
                 condition: succeeded()
               - template: /eng/pipelines/coreclr/templates/jit-exploratory-steps.yml
diff --git a/eng/pipelines/coreclr/templates/crossgen2-comparison-build-job.yml b/eng/pipelines/coreclr/templates/crossgen2-comparison-build-job.yml
index d91ed3f2febe3f..bf598beec35d35 100644
--- a/eng/pipelines/coreclr/templates/crossgen2-comparison-build-job.yml
+++ b/eng/pipelines/coreclr/templates/crossgen2-comparison-build-job.yml
@@ -72,7 +72,7 @@ jobs:
       value: $(productDirectory)$(dir)$(targetFlavor)$(dir)crossgen2$(dir)crossgen2.dll
     - ${{ if ne(parameters.archType, 'x64') }}:
       - name: crossgen2location
-        value: $(productDirectory)$(dir)$(targetFlavor)$(dir)x64$(dir)crossgen2$(dir)tools$(dir)crossgen2.dll
+        value: $(productDirectory)$(dir)$(targetFlavor)$(dir)x64$(dir)crossgen2$(dir)crossgen2.dll
     - name: librariesProductDllDir
       value: $(Build.SourcesDirectory)$(dir)artifacts$(dir)bin$(dir)runtime$(dir)net10.0-$(osGroup)$(osSubgroup)-$(buildConfig)-$(archType)
 
@@ -98,7 +98,7 @@ jobs:
         displayName: 'product build'
 
     # Populate Core_Root
-    - script: $(Build.SourcesDirectory)/src/tests/build$(scriptExt) $(buildConfig) $(archType) $(crossArg) generatelayoutonly /p:UsePublishedCrossgen2=false
+    - script: $(Build.SourcesDirectory)/src/tests/build$(scriptExt) $(buildConfig) $(archType) $(crossArg) generatelayoutonly
       displayName: Populate Core_Root
 
     # Create work item directory and populate with assemblies
diff --git a/eng/pipelines/coreclr/templates/crossgen2-comparison-job.yml b/eng/pipelines/coreclr/templates/crossgen2-comparison-job.yml
index c268335145008b..1183d2598a4804 100644
--- a/eng/pipelines/coreclr/templates/crossgen2-comparison-job.yml
+++ b/eng/pipelines/coreclr/templates/crossgen2-comparison-job.yml
@@ -94,7 +94,7 @@ jobs:
         displayName: 'product build'
 
     # Populate Core_Root
-    - script: $(Build.SourcesDirectory)/src/tests/build$(scriptExt) $(buildConfig) $(archType) $(crossArg) generatelayoutonly /p:UsePublishedCrossgen2=false
+    - script: $(Build.SourcesDirectory)/src/tests/build$(scriptExt) $(buildConfig) $(archType) $(crossArg) generatelayoutonly
       displayName: Populate Core_Root
 
     - task: DownloadPipelineArtifact@2
diff --git a/eng/pipelines/coreclr/templates/perf-job.yml b/eng/pipelines/coreclr/templates/perf-job.yml
index 047e583765489a..757b35aa5e4b71 100644
--- a/eng/pipelines/coreclr/templates/perf-job.yml
+++ b/eng/pipelines/coreclr/templates/perf-job.yml
@@ -305,7 +305,7 @@ jobs:
           checkDownloadedFiles: true
 
     # Create Core_Root
-    - script: $(Build.SourcesDirectory)/src/tests/build$(scriptExt) $(buildConfig) $(archType) generatelayoutonly $(librariesOverrideArg) $(_crossBuildPropertyArg) /p:UsePublishedCrossgen2=false
+    - script: $(Build.SourcesDirectory)/src/tests/build$(scriptExt) $(buildConfig) $(archType) generatelayoutonly $(librariesOverrideArg) $(_crossBuildPropertyArg)
       displayName: Create Core_Root
       condition: and(succeeded(), ne(variables.runtimeFlavorName, 'Mono'), not(in('${{ parameters.runtimeType }}', 'wasm', 'AndroidMono', 'iOSMono', 'iOSNativeAOT')))
 
diff --git a/eng/pipelines/coreclr/templates/superpmi-collect-job.yml b/eng/pipelines/coreclr/templates/superpmi-collect-job.yml
index 0f33e1d29b38be..5d9c397a892f71 100644
--- a/eng/pipelines/coreclr/templates/superpmi-collect-job.yml
+++ b/eng/pipelines/coreclr/templates/superpmi-collect-job.yml
@@ -85,6 +85,6 @@ jobs:
           displayName: 'generic managed test artifacts'
 
     # Create Core_Root
-    - script: $(Build.SourcesDirectory)/src/tests/build$(scriptExt) $(buildConfig) $(archType) $(crossArg) generatelayoutonly $(librariesOverrideArg) /p:UsePublishedCrossgen2=false
+    - script: $(Build.SourcesDirectory)/src/tests/build$(scriptExt) $(buildConfig) $(archType) $(crossArg) generatelayoutonly $(librariesOverrideArg)
       displayName: Create Core_Root
       condition: succeeded()
diff --git a/src/tests/Common/Directory.Build.targets b/src/tests/Common/Directory.Build.targets
index eb2d22c557fb55..ca3cd3786b9713 100644
--- a/src/tests/Common/Directory.Build.targets
+++ b/src/tests/Common/Directory.Build.targets
@@ -19,7 +19,6 @@
     <CopyCoreDisToolsToCoreRoot Condition="$(GCStressDependsOnCoreDisTools) And '$(DotNetBuildSourceOnly)' != 'true'">true</CopyCoreDisToolsToCoreRoot>
     <!-- Non-desktop OS's use a custom dotnet host, instead of corerun -->
     <IsDesktopOS Condition="'$(TargetsBrowser)' != 'true' and '$(TargetsAndroid)' != 'true' and '$(TargetstvOS)' != 'true' and '$(TargetsiOS)' != 'true' and '$(TargetsMacCatalyst)' != 'true'">true</IsDesktopOS>
-    <UsePublishedCrossgen2 Condition="'$(RuntimeFlavor)' == 'coreclr' and '$(TestBuildMode)' != 'nativeaot'">true</UsePublishedCrossgen2>
   </PropertyGroup>
 
   <Import Project="$(RepositoryEngineeringDir)coredistools.targets" Condition="$(CopyCoreDisToolsToCoreRoot)" />
@@ -38,46 +37,6 @@
   <Target Name="CopyDependencyToCoreRoot"
     DependsOnTargets="ResolveAssemblyReferences;ResolveRuntimeFilesFromLocalBuild">
 
-    <!-- Copy apphost to crossgen2_publish directory.
-        The default configuration between product and tests are flipped. When we build project like:
-        `build -c debug -rc checked`, the corresponding test command is `src/tests/build -checked -p:LibrariesConfiguration=debug`,
-        instead of `-debug -p:RuntimeConfiguration=checked`. That forces us to either pass the
-        `HostConfiguration=debug` explicitly or fix this disparity; both of which will break the dev workflow.
-
-        As a workaround, we will first check if the directory pointed by `HostConfiguration` exists, then check
-        `LibrariesConfiguration`.
-    -->
-
-    <PropertyGroup Condition="'$(UsePublishedCrossgen2)' == 'true'">
-      <_targetOS>$(TargetOS)</_targetOS>
-      <_targetOS Condition="'$(_targetOS)' == 'windows'">win</_targetOS>
-      <_apphostPath Condition="Exists('$(ArtifactsBinDir)$(_targetOS)-$(TargetArchitecture).$(HostConfiguration)\corehost\apphost$(ExeSuffix)')">$(ArtifactsBinDir)$(_targetOS)-$(TargetArchitecture).$(HostConfiguration)\corehost\apphost$(ExeSuffix)</_apphostPath>
-      <_apphostPath Condition="'$(_apphostPath)' == '' and Exists('$(ArtifactsBinDir)$(_targetOS)-$(TargetArchitecture).$(LibrariesConfiguration)\corehost\apphost$(ExeSuffix)')">$(ArtifactsBinDir)$(_targetOS)-$(TargetArchitecture).$(LibrariesConfiguration)\corehost\apphost$(ExeSuffix)</_apphostPath>
-      <_toolsConfiguration Condition="Exists('$(ArtifactsBinDir)ILLink.Tasks\$(ToolsConfiguration)\$(NetCoreAppToolCurrent)\ILLink.Tasks.dll')">$(ToolsConfiguration)</_toolsConfiguration>
-      <_toolsConfiguration Condition="Exists('$(ArtifactsBinDir)ILLink.Tasks\$(LibrariesConfiguration)\$(NetCoreAppToolCurrent)\ILLink.Tasks.dll')">$(LibrariesConfiguration)</_toolsConfiguration>
-    </PropertyGroup>
-
-    <MakeDir Condition="'$(UsePublishedCrossgen2)' == 'true'" Directories="$(ArtifactsObjDir)coreclr\crossgen2_publish\$(TargetOS).$(TargetArchitecture).$(RuntimeConfiguration)" />
-
-    <Copy
-      SourceFiles="$(_apphostPath)"
-      DestinationFiles="$(ArtifactsObjDir)coreclr\crossgen2_publish\$(TargetOS).$(TargetArchitecture).$(RuntimeConfiguration)\apphost$(ExeSuffix)"
-      Condition="'$(UsePublishedCrossgen2)' == 'true'" />
-
-    <!-- Publish crossgen2 on supported platforms. -->
-
-    <MSBuild Condition="'$(UsePublishedCrossgen2)' == 'true'"
-      Targets="Restore"
-      BuildInParallel="true"
-      Properties="NativeAotSupported=$(NativeAotSupported);UseNativeAotForComponents=$(UseNativeAotForComponents);ToolsConfiguration=$(_toolsConfiguration);MSBuildRestoreSessionId=$([System.Guid]::NewGuid())"
-      Projects="$(InstallerProjectRoot)pkg\sfx\Microsoft.NETCore.App\Microsoft.NETCore.App.Crossgen2.sfxproj" />
-
-    <MSBuild Condition="'$(UsePublishedCrossgen2)' == 'true'"
-      Targets="PublishToDisk"
-      BuildInParallel="true"
-      Properties="NativeAotSupported=$(NativeAotSupported);UseNativeAotForComponents=$(UseNativeAotForComponents);ToolsConfiguration=$(_toolsConfiguration);OutputPath=$(CORE_ROOT)\crossgen2;LibrariesConfiguration=$(LibrariesConfiguration)"
-      Projects="$(InstallerProjectRoot)pkg\sfx\Microsoft.NETCore.App\Microsoft.NETCore.App.Crossgen2.sfxproj" />
-
     <ItemGroup>
       <RunTimeDependencyCopyLocal Include="@(RuntimeCopyLocalItems)" />
       <RunTimeDependencyCopyLocal Include="@(NativeCopyLocalItems)" />
@@ -123,7 +82,7 @@
       <RunTimeArtifactsIncludeFolders Include="IL/" TargetDir="IL/" />
 
       <!-- Used for Crossgen2 R2R tests -->
-      <RunTimeArtifactsIncludeFolders Include="crossgen2/" TargetDir="crossgen2/" Condition="'$(UsePublishedCrossgen2)' != 'true'">
+      <RunTimeArtifactsIncludeFolders Include="crossgen2-published/" TargetDir="crossgen2/">
         <IncludeSubFolders>True</IncludeSubFolders>
       </RunTimeArtifactsIncludeFolders>
 
diff --git a/src/tests/build.proj b/src/tests/build.proj
index 00ca79e181365d..96b6595c4ba91e 100644
--- a/src/tests/build.proj
+++ b/src/tests/build.proj
@@ -679,7 +679,7 @@
       <CrossgenCmd Condition="'$(__CompositeBuildMode)' == ''">$(CrossgenCmd) --crossgen2-parallelism 1</CrossgenCmd>
 
       <CrossgenCmd>$(CrossgenCmd) --verify-type-and-field-layout</CrossgenCmd>
-      <CrossgenCmd>$(CrossgenCmd) --crossgen2-path "$(__BinDir)\$(BuildArchitecture)\crossgen2\tools\crossgen2.dll"</CrossgenCmd>
+      <CrossgenCmd>$(CrossgenCmd) --crossgen2-path "$(__BinDir)\$(BuildArchitecture)\crossgen2\crossgen2.dll"</CrossgenCmd>
     </PropertyGroup>
 
     <Message Importance="High" Text="$(MsgPrefix)Compiling framework using Crossgen2: $(CrossgenCmd)" />