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workflow:
class: Workflow
cwlVersion: v1.0
id: test
label: test
$namespaces:
sbg: 'https://www.sevenbridges.com/'
inputs:
- id: sample
type: 'string[]?'
default:
- sample1
'sbg:x': 0
'sbg:y': 532.4375
- id: fastq1
type: 'File[]'
default:
- class: File
path: ./sample1.raw_1.fq.gz
'sbg:x': 0
'sbg:y': 853.015625
- id: fastq2
type: 'File[]?'
default:
- class: File
path: ./sample1.raw_2.fq.gz
'sbg:x': 0
'sbg:y': 746.15625
outputs: []
steps:
- id: cutadaptparallel_v2
in:
- id: fastq1
source:
- fastq1
- id: fastq2
source:
- fastq2
- id: sample
source:
- sample
out:
- id: outfastq1
- id: outfastq2
run:
class: Workflow
cwlVersion: v1.0
id: cutadaptparallel_v2
label: cutadaptparallel_v2
$namespaces:
sbg: 'https://www.sevenbridges.com/'
inputs:
- id: fastq1
type: 'File[]?'
'sbg:x': -323.37908935546875
'sbg:y': -315.423583984375
- id: fastq2
type: 'File[]?'
'sbg:x': -323.4378662109375
'sbg:y': -445.9402160644531
- id: sample
type: 'string[]?'
'sbg:x': -324.03839111328125
'sbg:y': -556.1343383789062
outputs:
- id: outfastq1
outputSource:
- cutadapt_v2/outfastq1
type: 'File[]?'
'sbg:x': 90.7265625
'sbg:y': -422
- id: outfastq2
outputSource:
- cutadapt_v2/outfastq2
type: 'File[]?'
'sbg:x': 36
'sbg:y': -564
steps:
- id: cutadapt_v2
in:
- id: fastq1
source: fastq1
- id: fastq2
source: fastq2
- id: sample
source: sample
out:
- id: outfastq1
- id: outfastq2
run:
class: CommandLineTool
cwlVersion: v1.0
$namespaces:
sbg: 'https://www.sevenbridges.com/'
id: cutadapt_v2
baseCommand:
- cutadapt
inputs:
- id: fastq1
type: File
inputBinding:
position: 4
- default: AGATCGGAAGAGC
id: adapter
type: string?
inputBinding:
position: 1
prefix: '-b'
- default: $(inputs.sample).cutadapt.fq1.gz
id: output
type: string?
inputBinding:
position: 2
prefix: '-o'
valueFrom: $(inputs.sample).cutadapt.fq1.gz
- default: $(inputs.sample).cutadapt.fq2.gz
id: paired_output
type: string?
inputBinding:
position: 3
prefix: '-p'
valueFrom: $(inputs.sample).cutadapt.fq2.gz
- id: fastq2
type: File?
inputBinding:
position: 5
- id: sample
type: string?
outputs:
- id: outfastq1
type: File?
outputBinding:
glob: |
$(inputs.sample).cutadapt.fq1.gz
- id: outfastq2
type: File?
outputBinding:
glob: |
$(inputs.sample).cutadapt.fq2.gz
doc: >-
cutadapt -b AGATCGGAAGAGC -o 00.rawdata/sample1.raw_1.fq -p
00.rawdata/sample1.raw_2.fq
/gluster/home/micro/Pipeline/16S_pipeline_V3.0/example/00.data/sample1.raw_1.fq.gz
/gluster/home/micro/Pipeline/16S_pipeline_V3.0/example/00.data/sample1.raw_2.fq.gz
>00.rawdata/cutadapt.log
label: cutadapt-v2
hints:
- class: DockerRequirement
dockerPull: 'cutadapt:1.16'
requirements:
- class: InlineJavascriptRequirement
label: cutadapt-v2
scatter:
- fastq1
- fastq2
- sample
scatterMethod: dotproduct
'sbg:x': -88.69266510009766
'sbg:y': -470.6273193359375
requirements:
- class: ScatterFeatureRequirement
label: cutadaptparallel_v2
'sbg:x': 540.9274291992188
'sbg:y': 677.3812255859375
requirements:
- class: SubworkflowFeatureRequirement
If the image is created using "cwltool.Dockerfile", the following error message is displayed:
INFO [job cutadapt_v2] Max memory used: 6MiB
INFO [job cutadapt_v2] completed success
INFO [step cutadapt_v2] completed success
INFO [workflow cutadaptparallel_v2] completed success
INFO [step cutadaptparallel_v2] completed success
ERROR Unhandled exception
Traceback (most recent call last):
File "/usr/local/lib/python3.10/site-packages/cwltool/workflow_job.py", line 777, in try_make_job
yield from jobs
File "/usr/local/lib/python3.10/site-packages/cwltool/workflow_job.py", line 76, in job
yield from self.step.job(joborder, output_callback, runtimeContext)
RuntimeError: close method on generator classes unimplemented
However, if use cwLTool installed directly by Conda, it will work without error
The file used for the test:
test.zip
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